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Fmoc Solid Phase Peptide Synthesis: A Practical Approach by Peter D. White, W. C. Chan

Fmoc Solid Phase Peptide Synthesis: A Practical Approach Peter D. White, W. C. Chan ebook
ISBN: 0199637249, 9780199637249
Format: pdf
Page: 376
Publisher: Oxford University Press, USA

Return to citation in text: [1] [2]; Chan, W. Automated solid-phase peptide synthesis coupled with optimized Fmoc (9-fluorenylmethoxy-carbonyl) chemistry to obtain peptides in high yield and purity is discussed. Polymerization on the rocks: beta-amino acids and arginine. [0168] (28) Atherton, E.; Sheppard, R. Doi:10.1016/S0040-4039(01)01102-9. This new approach offers significant advantages such as more simple utility, minimal side reactions, large scale synthesis of peptide drugs, and greater cost effectiveness. Fmoc solid phase peptide synthesis. Rink amide resin, 9-fluorenylmethoxycarbonyl (Fmoc) protected amino acid derivatives, and all the reagents for solid-phase peptide synthesis were purchased from Novabiochem (Laufelfingen, Switzerland). This peptide can be easily crystallised and is therefore well .. Fmoc Solid Phase Peptide Synthesis: A Practical Approach. Solid phase peptide synthesis--a practical approach; IRL Press: Oxford, England, 1989; pp 203 [0169] (29) Gausepohl, H.; Kral, M.; Boulin, C.; Frank R. Oxford University Press, Oxford. As thousands of individuals worldwide become involved with the study of peptides, and the demand for synthetic peptides rapidly increases, so too does the need for a practical, single-volume treatment of this growing field. In the years since the publication of Atherton and Sheppard's volume, the technique of Fmoc solid-phase peptide synthesis has matured considerably and is now the standard approach for the routine production of peptides. After Fmoc solid phase peptide synthesis, on-bead formylation and HPLC purification, more than 30 mg of the fully 13C/15N-labelled tripeptide 1 could be isolated in a typical batch. Fmoc Solid Phase Peptide Synthesis: A Practical Approach; Oxford University Press: New York, 2004. Peptide Synthesis Peptides were synthesized manually by solid phase method by stepwise coupling of Fmoc-amino acids to the growing chain on a Rink amide resin (0.9–1.1 mmol/g, Figures 1 and 3 for Desmopressin and Oxytocin) and 2-chlorotrityl chloride resin (Figure 2, for Octreotide). Side-chain protecting groups employed for peptide synthesis were as ..